HECTD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HECTD1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 20605-1-AP

Synonyme

HECT domain-containing protein 1, HECT domain containing 1, EULIR, EC:2.3.2.26, E3 ubiquitin-protein ligase HECTD1

---

• 

  WB analysis using 20605-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20605-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20605-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20605-1-AP (HECTD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20605-1-AP (HECTD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20605-1-AP

  Immunofluorescent analysis of Hela cells, using HECTD1 antibody 20605-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 2 publications below

Produktinformation

20605-1-AP bindet in WB, IHC, IF/ICC, ELISA HECTD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HECTD1 fusion protein Ag14581
• Vollständiger Name: HECT domain containing 1
• Berechnetes Molekulargewicht: 2612 aa, 290 kDa
• Beobachtetes Molekulargewicht: 290 kDa
• GenBank-Zugangsnummer: BC006237
• Gene symbol: HECTD1
• Gene ID (NCBI): 25831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This protein is specific to HECTD1.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Condensin I and II Complexes License Full Estrogen Receptor α-Dependent Enhancer Activation.; Authors - Wenbo Li
mouse	WB	J Neurosci; Circular RNA DLGAP4 ameliorates ischemic stroke outcomes by targeting miR-143 to regulate endothelial-mesenchymal transition associated with blood-brain barrier integrity.; Authors - Ying Bai
Mouse	WB	J Neurosci; Unbiased proteomics of early Lewy body formation model implicates active microtubule affinity-regulating kinases (MARKs) in synucleinopathies.; Authors - Michael X Henderson
mouse	IF,WB	Cell Biosci; Involvement of HECTD1 in LPS-induced astrocyte activation via σ-1R-JNK/p38-FOXJ2 axis.; Authors - Ying Tang ; KO Validated
mouse	WB	Cell Microbiol; The serine/threonine protein kinase of Streptococcus suis serotype 2 affects the ability of the pathogen to penetrate the blood brain barrier.; Authors - Liu Rui
human	WB	Hum Cell; Nucleoporin 93, a new substrate of the E3 ubiquitin protein ligase HECTD1, promotes esophageal squamous cell carcinoma progression; Authors - Jinfeng Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Nick (Verified Customer) (04-20-2022)

Excellent stuff. Highly recommend.

• Applications: Western Blot, Immunoprecipitation