HIRIP3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HIRIP3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14992-1-AP

Synonyme

HIRA-interacting protein 3

---

• 

  WB analysis of mouse skeletal muscle using 14992-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14992-1-AP

  WB result of HIRIP3 antibody (14992-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIRIP3 transfected HeLa cells.

• 

  WB analysis of human heart using 14992-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 14992-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14992-1-AP

  IP result of anti-HIRIP3 (IP:14992-1-AP, 4ug; Detection:14992-1-AP 1:3000) with HEK-293 cells lysate 1600 ug.

• 

  IHC staining of human ovary using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 14992-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HeLa-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14992-1-AP bindet in WB, IHC, IP, ELISA HIRIP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIRIP3 fusion protein Ag6978
• Vollständiger Name: HIRA interacting protein 3
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 69-90 kDa
• GenBank-Zugangsnummer: BC000588
• Gene symbol: HIRIP3
• Gene ID (NCBI): 8479
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.