Histone H2B Polyklonaler Antikörper

Histone H2B Polyklonal Antikörper für WB, IHC, IF, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (2)

Kat-Nr. : 15857-1-AP

Synonyme

HIST2H2BE, GL105, H2B, H2B.1, H2B/q

---

• 

  WB analysis using 15857-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15857-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15857-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15857-1-AP

  IP result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug.

• 

  IHC staining of human prostate cancer using 15857-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15857-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15857-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15857-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining using 15857-1-AP

  Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausnierengewebe, Maus-Pankreasgewebe, Rattennierengewebe, U2OS-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: N/A

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IF: See 5 publications below

Produktinformation

15857-1-AP bindet in WB, IHC, IF, IP, ELISA Histone H2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H2B fusion protein Ag7811
• Vollständiger Name: histone cluster 2, H2be
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14-17 kDa
• GenBank-Zugangsnummer: BC005827
• Gene symbol: Histone H2B
• Gene ID (NCBI): 8349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone H2B is a core component of the nucleosome, the fundamental unit of chromatin. It forms a dimer with Histone H2A, and two H2A-H2B dimers combine with a (H3-H4)₂ tetramer to create the histone octamer. The primary function of this structure is to package DNA into a compact and manageable form. However, H2B is also subject to various post-translational modifications (PTMs), such as ubiquitination, which serve as epigenetic marks to regulate gene expression by altering chromatin accessibility.

Publikationen

Species	Application	Title
human	IF	Nat Nanotechnol; Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.; Authors - Tanmoy Saha
human	IF	Nat Biomed Eng; Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues; Authors - Su-Jin Heo
human	WB	Oncogene; HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.; Authors - Laura Monteonofrio
human	WB	Elife; Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia.; Authors - William F Richter
human	WB	Mol Cell Proteomics; Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.; Authors - Rui Sun
human	WB	Cell Discov; USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.; Authors - Jialun Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Gunjita (Verified Customer) (01-06-2020)

N/A

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hek293

FH

Aurelie (Verified Customer) (11-19-2018)

strong signal around 20-25kda in mouse cortex (25µg) at 1:1000, (1:2000 should be ok as well)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: mouse cortex