HKR1 Polyklonaler Antikörper

HKR1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14902-1-AP

Synonyme

GLI Kruppel family member HKR1, HKR1, Protein HKR1

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 14902-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HEK-293 using 14902-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14902-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14902-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14902-1-AP bindet in WB, ELISA HKR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HKR1 fusion protein Ag6694
• Vollständiger Name: GLI-Kruppel family member HKR1
• Berechnetes Molekulargewicht: 73 kDa, 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC053845
• Gene symbol: HKR1
• Gene ID (NCBI): 284459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HKR1, also named as ZNF875, belongs to the krueppel C2H2-type zinc-finger protein family. HRK1 is a modular protein that comprises a photosensory rhodopsin, a histidine kinase, a response regulator and a putative guanylyl cyclase domain. HKR1 is related to the protonation state of the Schiff base function that provides the linkage of the retinal chromophore with a Lys side chain of the protein (PMID: 25836735). HRK1 has 2 isoforms with the molecular mass of 73-75 kDa.