HLA class I ABC Monoklonaler Antikörper

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• KD/KO Validated

HLA class I ABC Monoklonal Antikörper für WB, IHC, IF/ICC, FC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, FC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

5C5B7

Kat-Nr. : 66013-1-Ig

Synonyme

HLA-A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I, HLA A

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  WB analysis using 66013-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of U-937 using 66013-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66013-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66013-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66013-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66013-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human white blood using 66013-1-Ig

  human white blood cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 66013-1-Ig

  pig spleen tissue was subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66013-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 66013-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Raji using 66013-1-Ig

  1X10^6 Raji cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (66013-1-Ig, Clone:5C5B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NCCIT-Zellen, Hausschwein-Milzgewebe, Raji-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

66013-1-Ig bindet in WB, IHC, IF/ICC, FC, IP, ELISA HLA class I ABC und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HLA class I ABC fusion protein Ag7488
• Vollständiger Name: major histocompatibility complex, class I, A
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40-44 kDa
• GenBank-Zugangsnummer: BC003069
• Gene symbol: HLA-A
• Gene ID (NCBI): 3105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Publikationen

Species	Application	Title
human	IF	Front Med; Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis; Authors - Weiwei Xian
		Oncotarget; Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells.; Authors - Jianlong Li
human	WB	J Tissue Eng Regen Med; Sequential hydrophile and lipophile solubilization as an efficient method for decellularization of porcine aortic valve leaflets: structure, mechanical property and biocompatibility study.; Authors - Wei-Hua Qiao
human	WB	J Biol Chem; The transferrin receptor CD71 regulates type II CD38, revealing tight topological compartmentalization of intracellular cyclic ADP-ribose production.; Authors - Qi Wen Deng ; KD Validated
pig	IHC	Tissue Eng Part A; DECELLULARIZATION OF MASSIVE BONE ALLOGRAFTS BY PERFUSION: A NEW PROTOCOL FOR TISSUE ENGINEERING; Authors - Robin Evrard
human	IHC	Asian Pac J Cancer Prev; Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.; Authors - Zhao Xiaogang X