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HLA class I (HLA-C) Polyklonaler Antikörper

HLA class I (HLA-C) Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15777-1-AP

Synonyme

D6S204, HLA C, HLA class I (HLA-C), HLA Cw, HLA Cw12, HLA JY3, HLAC, HLA-C, HLC C, MHC class I antigen Cw*7, PSORS1

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    WB analysis using 15777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IP experiment of Jurkat using 15777-1-AP

    IP Result of anti-HLA class I (HLA-C) (IP:15777-1-AP, 4ug; Detection:15777-1-AP 1:1000) with Jurkat cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IHC staining of human lymphoma using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IHC staining of human lymphoma using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IF Staining of HeLa using 15777-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHL-60-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
Erfolgreiche IPJurkat-Zellen
Erfolgreiche Detektion in IHChumanes Lymphomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

15777-1-AP bindet in WB, IP, IHC, IF, ELISA HLA class I (HLA-C) und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HLA class I (HLA-C) fusion protein Ag8461
Vollständiger Name major histocompatibility complex, class I, C
Berechnetes Molekulargewicht 366 aa, 41 kDa
Beobachtetes Molekulargewicht 44 kDa
GenBank-ZugangsnummerBC007814
Gene symbol HLA-C
Gene ID (NCBI) 3107
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)

Protokolle

Produktspezifische Protokolle
WB protocol for HLA class I (HLA-C) antibody 15777-1-APProtokoll herunterladen
IHC protocol for HLA class I (HLA-C) antibody 15777-1-APProtokoll herunterladen
IF protocol for HLA class I (HLA-C) antibody 15777-1-APProtokoll herunterladen
IP protocol for HLA class I (HLA-C) antibody 15777-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

J Matern Fetal Neonatal Med

HLA-C promotes proliferation and cell cycle progression in trophoblast cells

Authors - Hong Lv
View Article