HLA class I (HLA-C) Polyklonaler Antikörper

HLA class I (HLA-C) Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15777-1-AP

Synonyme

HLA-C, HLAC, HLA JY3, HLA Cw12, HLA Cw

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  WB analysis using 15777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15777-1-AP

  IP result of anti-HLA class I (HLA-C) (IP:15777-1-AP, 4ug; Detection:15777-1-AP 1:1000) with Jurkat cells lysate 2400ug.

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  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15777-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HLA class I (HLA-C) antibody (15777-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15777-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA, Cell treatment HLA class I (HLA-C) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLA class I (HLA-C) fusion protein Ag8461
• Vollständiger Name: major histocompatibility complex, class I, C
• Berechnetes Molekulargewicht: 366 aa, 41 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC007814
• Gene symbol: HLA class I (HLA-C)
• Gene ID (NCBI): 3107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)

Publikationen

Species	Application	Title
human	WB	J Matern Fetal Neonatal Med; HLA-C promotes proliferation and cell cycle progression in trophoblast cells; Authors - Hong Lv
human	IHC	Front Pharmacol; Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis; Authors - Yanling Wang
human	Cell treatment	Nature; Shifts in receptors during submergence of an encephalitic arbovirus; Authors - Wanyu Li
mouse	IF	Neuroscience; HLA is a potent immunoinflammatory target in asymptomatic Alzheimer's disease; Authors - Yingwei Zheng
mouse	WB	Nat Commun; Neoantigen enriched biomimetic nanovaccine for personalized cancer immunotherapy; Authors - Yuwei Li