HLA-DMB Polyklonaler Antikörper

HLA-DMB Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 21704-1-AP

Synonyme

RING7, MHC class II antigen DMB, HLA DMB, DMB

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  WB analysis using 21704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21704-1-AP (HLA-DMB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21704-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21704-1-AP (HLA-DMB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21704-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21704-1-AP (HLA-DMB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 21704-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DMB antibody (21704-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

21704-1-AP bindet in WB, IHC, IF/ICC, ELISA HLA-DMB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLA-DMB fusion protein Ag16320
• Vollständiger Name: major histocompatibility complex, class II, DM beta
• Berechnetes Molekulargewicht: 263 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC027175
• Gene symbol: HLA-DMB
• Gene ID (NCBI): 3109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes; Authors - Rupert L Mayer
human	WB,IF	Inflammation; RNA-seq Based Transcriptome Analysis Reveals Role of Myoglobin in Rheumatoid Arthritis; Authors - Haibin Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Adillah (Verified Customer) (04-20-2023)

good for western blot

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000