HLA-DR Monoklonaler Antikörper

HLA-DR Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

L243

Kat-Nr. : 65218-1-Ig

Synonyme

HLA DRA, HLA DRB, HLA-DR, HLA-DRA, HLA-DRB, MHC class II antigen DRA

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  FC experiment of human PBMCs using 65218-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human HLA-DR (65218-1-Ig, Clone:L243) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65218-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human HLA-DR (65218-1-Ig, Clone:L243) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

65218-1-Ig bindet in IF, FC HLA-DR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Vollständiger Name: major histocompatibility complex, class II, DR alpha
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC032350
• Gene symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Publikationen

Species	Application	Title
human	IF	J Neuroinflammation; Astrocytic lipid droplets contain MHCII and may act as cogs in the antigen presentation machinery; Authors - Chiara Beretta