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HLA-E Polyklonaler Antikörper

HLA-E Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15008-1-AP

Synonyme

EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of THP-1 cells using HLA-E Polyclonal antibody (15008-1-AP)

    WB analysis of THP-1 using 15008-1-AP

    WB result of HLA-E antibody (15008-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-E transfected THP-1 cells.

  • Western Blot (WB) analysis of THP-1 cells using HLA-E Polyclonal antibody (15008-1-AP)

    WB analysis of THP-1 using 15008-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using HLA-E Polyclonal antibody (15008-1-AP)

    WB analysis of HUVEC using 15008-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using HLA-E Polyclonal antibody (15008-1-AP)

    WB analysis of human kidney using 15008-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using HLA-E Polyclonal antibody (15008-1-AP)

    WB analysis of human liver using 15008-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBTHP-1-Zellen, humanes Lebergewebe, humanes Nierengewebe, HUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15008-1-AP bindet in WB, ELISA HLA-E und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HLA-E fusion protein Ag6703
Vollständiger Name major histocompatibility complex, class I, E
Berechnetes Molekulargewicht 40 kDa
Beobachtetes Molekulargewicht 40 kDa
GenBank-ZugangsnummerBC002578
Gene symbol HLA-E
Gene ID (NCBI) 3133
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)

Protokolle

Produktspezifische Protokolle
WB protocol for HLA-E antibody 15008-1-APProtokoll herunterladen
Standard-Protokolle
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