HLA-E Polyklonaler Antikörper

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HLA-E Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15008-1-AP

Synonyme

HLA 6.2, HLA E, HLA-6.2, HLAE, MHC class I antigen E

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  WB analysis using 15008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 15008-1-AP

  WB result of HLA-E antibody (15008-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-E transfected THP-1 cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, Raji-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15008-1-AP bindet in WB, ELISA HLA-E und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLA-E fusion protein Ag6703
• Vollständiger Name: major histocompatibility complex, class I, E
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC002578
• Gene symbol: HLA-E
• Gene ID (NCBI): 3133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)