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HLTF Polyklonaler Antikörper
HLTF Polyklonal Antikörper für WB, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14286-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, K-562-Zellen |
| Erfolgreiche IP | HeLa-Zellen |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Produktinformation
14286-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA HLTF und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | HLTF fusion protein Ag5596 |
| Vollständiger Name | helicase-like transcription factor |
| Berechnetes Molekulargewicht | 114 kDa |
| Beobachtetes Molekulargewicht | 114 kDa |
| GenBank-Zugangsnummer | BC044659 |
| Gene symbol | HLTF |
| Gene ID (NCBI) | 6596 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Helicase-like Transcription Factor (HLTF) is a crucial nuclear protein and member of the SWI/SNF family of chromatin-remodeling complexes, playing a dual role in transcription regulation and DNA damage repair. It functions dually as a regulator of gene expression, utilizing its ATP-dependent helicase activity to modify chromatin structure and facilitate transcription, and as a central effector in the DNA damage response pathway. HLTF is particularly crucial for promoting error-free DNA repair at stalled replication forks via a mechanism called template switching, which prevents the accumulation of mutagenic lesions. HLTF can be detected as 114 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for HLTF antibody 14286-1-AP | Protokoll herunterladen |
| IF protocol for HLTF antibody 14286-1-AP | Protokoll herunterladen |
| IP protocol for HLTF antibody 14286-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Elife Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response. | ||
Front Immunol Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses. | ||
Int J Oncol HLTF suppresses the migration and invasion of colorectal cancer cells via TGF‑β/SMAD signaling in vitro.
| ||
Oncogenesis HLTF promotes hepatocellular carcinoma progression by enhancing SRSF1 stability and activating ERK/MAPK pathway
| ||
Cell Rep A rewiring of DNA replication mediated by MRE11 exonuclease underlies primed-to-naive cell de-differentiation
|
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alexandra (Verified Customer) (07-12-2024) | Work perfectly not only with overexpressed protein, but with endogenous ones as well.
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