HMCN1 Polyklonaler Antikörper

HMCN1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 18837-1-AP

Synonyme

ARMD1, FBLN6, FIBL 6, FIBL6, Fibulin 6, hemicentin 1, HMCN1

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  WB analysis of mouse skin using 18837-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 18837-1-AP (HMCN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18837-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 18837-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 using 18837-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 18837-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18837-1-AP bindet in WB, IHC, IF/ICC, ELISA HMCN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: hemicentin 1
• Berechnetes Molekulargewicht: 613 kDa, 600 kDa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_031935
• Gene symbol: HMCN1
• Gene ID (NCBI): 83872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-976 amino acids of human HMCN1 can recognize all three isoforms of HMCN1.

Publikationen

Species	Application	Title
rat	WB	Front Pharmacol; Artemisinin relieves myocardial ischemia-reperfusion injury via modulating miR-29b-3p and hemicentin 1; Authors - Junyu Han
mouse	IHC	Front Cell Dev Biol; Hemicentin-1 is an essential extracellular matrix component during tooth root formation by promoting mesenchymal cells differentiation; Authors - Yujia Cui
mouse	IHC	PLoS Pathog; Reduction of antisense transcription affects bovine leukemia virus replication and oncogenesis; Authors - Thomas Joris
human	IF	J Exp Med; HMCN1 variants aggravate epidermolysis bullosa simplex phenotype; Authors - Shir Bergson