HMGCL Rekombinanter Antikörper

HMGCL Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242224F9

Kat-Nr. : 84769-4-RR

Synonyme

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9

---

• 

  WB analysis using 84769-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84769-4-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 84769-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGCL Recombinant antibody (84769-4-RR, Clone:242224F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 84769-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84769-4-RR against Human HMGCL were performed. The affinity constant is 1.13 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausnierengewebe, Rattennierengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84769-4-RR bindet in WB, FC (Intra), ELISA HMGCL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HMGCL fusion protein Ag10426
• Vollständiger Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
• Berechnetes Molekulargewicht: 325 aa, 34 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC010570
• Gene symbol: HMGCL
• Gene ID (NCBI): 3155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.