HO-1/HMOX1 Monoklonaler Antikörper

HO-1/HMOX1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D10A5

Kat-Nr. : CL488-66743

Synonyme

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1/HMOX1, HSP32

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Galerie der Validierungsdaten

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  IF Staining of human liver using CL488-66743

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL488-66743 (HO-1/HMOX1 antibody) at dilution of 1:100.

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  IF Staining of human liver using CL488-66743

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL488-66743 (HO-1/HMOX1 antibody) at dilution of 1:100.

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  IF Staining of mouse spleen using CL488-66743

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 HO-1/HMOX1 antibody (CL488-66743, Clone: 2D10A5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-66743

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HO-1/HMOX1 (CL488-66743, Clone:2D10A5) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Lebergewebe, Mausmilzgewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66743 bindet in IF, FC (Intra) HO-1/HMOX1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HO-1/HMOX1 fusion protein Ag21296
• Vollständiger Name: heme oxygenase (decycling) 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC001491
• Gene symbol: HMOX1
• Gene ID (NCBI): 3162
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C