HOMER1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HOMER1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 12433-1-AP

Synonyme

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, HOMER1, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves 1

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 12433-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:5000 incubated at room temperature for 1 hour.

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  WB analysis of human/mouse brain using 12433-1-AP

  WB result of anti-HOMER1 (12433-1-ap) with mouse brain lysate and human brain lysate.

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  WB analysis of NIH/3T3 using 12433-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 12433-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12433-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of fetal human brain using 12433-1-AP

  IP result of anti-HOMER1 (IP:12433-1-AP, 4ug; Detection:12433-1-AP 1:1000) with fetal human brain tissue lysate 4000 ug.

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  IHC staining of human gliomas using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12433-1-AP (HOMER1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12433-1-AP (HOMER1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 12433-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 12433-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 12433-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 12433-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human HOMER1 (12433-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, humanes Hirngewebe
• Erfolgreiche IP: fetales humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Pankreasgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

12433-1-AP bindet in WB, IP, IHC, IF, FC, ELISA HOMER1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOMER1 fusion protein Ag3109
• Vollständiger Name: homer homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 46-48 kDa
• GenBank-Zugangsnummer: BC015502
• Gene symbol: HOMER1
• Gene ID (NCBI): 9456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.

Publikationen

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
mouse	WB	Elife; Pathway-specific dysregulation of striatal excitatory synapses by LRRK2 mutations.; Authors - Chuyu Chen
mouse	IF	PLoS Genet; Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid.; Authors - Maha El Shahawy
rat	IF	Aging (Albany NY); Liraglutide preconditioning attenuates myocardial ischemia/ reperfusion injury via homer1 activation.; Authors - Xiangrong Cui ; KD Validated
Mouse, Rat	WB	CNS Neurosci Ther; Acid-sensing Ion Channels Activation and Hypoxia Upregulate Homer1a Expression.; Authors - Jing-Jing Su
mouse	WB,IF	Cell Signal; Tissue kallikrein protects neurons from hypoxia/reoxygenation-induced cell injury through Homer1b/c.; Authors - Su Jingjing J