Haptoglobin Polyklonaler Antikörper

Haptoglobin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 16665-1-AP

Synonyme

HP, BP, HAPT, HP2 ALPHA 2, HPA1S

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  WB analysis using 16665-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16665-1-AP

  Human plasma were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mouse serum, human plasma, rat plasma
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

16665-1-AP bindet in WB, IHC, IF, ELISA Haptoglobin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Haptoglobin fusion protein Ag10143
• Vollständiger Name: haptoglobin
• Berechnetes Molekulargewicht: 281aa,31 kDa; 228aa,25 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC058031
• Gene symbol: HP
• Gene ID (NCBI): 3240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn's disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).

Publikationen

Species	Application	Title
mouse	WB	Circ Res; Haptoglobin enhances cardiac transplant rejection.; Authors - Hua Shen
mouse	WB	Theranostics; Interleukin-6 Induced "Acute" Phenotypic Microenvironment Promotes Th1 Anti-Tumor Immunity in Cryo-Thermal Therapy Revealed By Shotgun and Parallel Reaction Monitoring Proteomics.; Authors - Ting Xue
human	IHC	Clin Transl Med; Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.; Authors - Qian Hao
		Antioxid Redox Signal; Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate.; Authors - John D Belcher
human	WB	World J Gastroenterol; N-linked glycoproteomic profiling in esophageal squamous cell carcinoma; Authors - Qi-Wei Liu
human	IHC	Oncol Rep; Proteomic-based analysis for identification of potential serum biomarkers in gallbladder cancer.; Authors - Tan Yi Y