HPRT1 Polyklonaler Antikörper

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• KD/KO Validated

HPRT1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 15059-1-AP

Synonyme

HGPRT, HGPRTase, HPRT, HPRT1

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Galerie der Validierungsdaten

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  WB analysis using 15059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15059-1-AP

  WB result of HPRT1 antibody (15059-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT1 transfected HEK-293 cells.

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  WB analysis of mouse liver using 15059-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15059-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15059-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15059-1-AP

  IP result of anti-HPRT1(IP:15059-1-AP, 4ug; Detection:15059-1-AP 1:6000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human brain using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT1 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT1 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 15059-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HPRT1 antibody (15059-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 15059-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HPRT1 (15059-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslebergewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, Y79-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Leberkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 26 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

15059-1-AP bindet in WB, IP, IHC, IF, FC, ELISA HPRT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HPRT1 fusion protein Ag7044
• Vollständiger Name: hypoxanthine phosphoribosyltransferase 1
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa
• GenBank-Zugangsnummer: BC000578
• Gene symbol: HPRT1
• Gene ID (NCBI): 3251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HPRT1, also named HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.; Authors - Xiaoyan Yang
rat	WB	J Clin Invest; ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.; Authors - Hidemichi Kouzu
mouse	WB	Cell Rep; Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways; Authors - Tatsuki Yasuda
human,mouse	WB	Br J Cancer; Blocking of oestrogen signals improves anti-tumour effect regardless of oestrogen receptor alpha expression in cancer cells; Authors - Nabeel Kajihara
human	WB	EMBO Rep; MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.; Authors - Gongwei Wu
mouse	WB	Elife; Aging is associated with increased brain iron through cortex-derived hepcidin expression.; Authors - Tatsuya Sato