HRD1/SYVN1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

HRD1/SYVN1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

2C8A7

Kat-Nr. : 67488-1-Ig

Synonyme

SYVN1, 2C8A7, EC:2.3.2.27, HRD1, KIAA1810

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  WB analysis using 67488-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67488-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67488-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67488-1-Ig

  WB result of HRD1/SYVN1 antibody (67488-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRD1/SYVN1 transfected HeLa cells.

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  IHC staining of human liver cancer using 67488-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67488-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67488-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRD1/SYVN1 antibody (67488-1-Ig, Clone: 2C8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

67488-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA HRD1/SYVN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HRD1/SYVN1 fusion protein Ag4456
• Vollständiger Name: synovial apoptosis inhibitor 1, synoviolin
• Berechnetes Molekulargewicht: 617 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68-76 kDa
• GenBank-Zugangsnummer: BC030530
• Gene symbol: HRD1
• Gene ID (NCBI): 84447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

Publikationen

Species	Application	Title
mouse	IF	Cell Mol Life Sci; S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.; Authors - Yifei Sun
mouse	WB	FASEB J; UFMylation of HRD1 regulates endoplasmic reticulum homeostasis; Authors - Hui Luo
	CoIP	Clin Exp Pharmacol Physiol; Attenuation of renal fibrosis in mice due to lack of bombesin receptor-activated protein homologue; Authors - Zhi Peng
mouse	WB	Heliyon; Quantitative proteomics reveals the mechanism of endoplasmic reticulum stress-mediated pulmonary fibrosis in mice; Authors - Heng Li
goat	WB	Int J Mol Sci; Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods; Authors - Zhipeng Sun
mouse	WB,IF	Eur J Pharmacol; Down-regulation of Hrd1 protects against myocardial ischemia-reperfusion injury by regulating PPARα to prevent oxidative stress, endoplasmic reticulum stress, and cellular apoptosis; Authors - Boyu Xia ; KD Validated