HSD3B2 Polyklonaler Antikörper

HSD3B2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15516-1-AP

Synonyme

3b HSD, HSD3B2, HSDB, HSDB3, HSDB3B, SDR11E2

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 15516-1-AP

  mouse testis tissue was subjected to SDS PAGE followed by western blot with 15516-1-AP (HSD3B2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 15516-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15516-1-AP (HSD3B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 15516-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15516-1-AP (HSD3B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche Detektion in IHC: Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

15516-1-AP bindet in WB, IHC, IF, ELISA HSD3B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD3B2 fusion protein Ag7782
• Vollständiger Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC038419
• Gene symbol: HSD3B2
• Gene ID (NCBI): 3284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in placenta and skin (PMID:1682237). HSD3B2 plays a crucial role in steroid hormone biosynthesis and is thus of particular interest in hormone dependent tumors such as prostate cancer. Compared with normal tissue cytoplasmic HSD3B2 staining was stronger in prostate cancers (PMID: 29803408). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).

Publikationen

Species	Application	Title
mouse	WB	J Hazard Mater; Tributyltin chloride (TBTCL) induces cell injury via dysregulation of endoplasmic reticulum stress and autophagy in Leydig cells; Authors - Pengchen Chen
human	WB	Oncotarget; Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase.; Authors - Liping Wen
rat	WB, IHC	Endocrinology; A dietary medium-chain fatty acid, decanoic acid, inhibits recruitment of Nur77 to the HSD3B2 promoter in vitro, and reverses endocrine and metabolic abnormalities in a rat model of polycystic ovary syndrome.; Authors - Bao Hui Lee
mouse	IF	Front Physiol; Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation.; Authors - Guillaume Richer
mouse	IHC	Reprod Biomed Online; Brain-derived neurotrophic factor expression in granulosa lutein cells.; Authors - Dominguez Miguel A MA
human	IHC	Int J Clin Exp Pathol; Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile.; Authors - Anli Tong