HSPA4 Polyklonaler Antikörper

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HSPA4 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 21206-1-AP

Synonyme

APG2, Heat shock 70 kDa protein 4, Heat shock 70-related protein APG-2, Heat shock protein family H member 2, hsp70RY

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  WB analysis using 21206-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21206-1-AP

  WB result of HSPA4 antibody (21206-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 21206-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21206-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21206-1-AP

  IP result of anti-HSPA4 (IP:21206-1-AP, 4ug; Detection:21206-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21206-1-AP

  Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody 21206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MCF-7 using 21206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HSPA4 antibody (21206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of C6 using 21206-1-AP

  1x10^6 C6 cells were intracellularly stained with 0.4 ug HSPA4 Polyclonal antibody (21206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, HEK-293-Zellen, Maushirngewebe, Maushodengewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• ELISA: See 1 publications below

Produktinformation

21206-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA HSPA4 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPA4 fusion protein Ag15581
• Vollständiger Name: heat shock 70kDa protein 4
• Berechnetes Molekulargewicht: 840 aa, 94 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC110861
• Gene symbol: HSPA4
• Gene ID (NCBI): 3308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (HSP110) subfamily of HSP70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors. This antibody well recognized the endogenous HSPA4 protein in mouse brain/ testis tissues. (PMID: 21487003)

Publikationen

Species	Application	Title
rat	WB	Int J Mol Sci; Comparative Hypothalamic Proteomic Analysis Between Diet-Induced Obesity and Diet-Resistant Rats; Authors - Pengjiao Xi
mouse	WB	Int J Biochem Cell Biol; Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.; Authors - Yang Yang
human	ELISA	Eur J Clin Invest; Salivary monitoring related to major surgery.; Authors - Ullmann Yehuda Y
	WB	Int J Mol Sci; Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1.; Authors - Lin Liu
mouse	WB	Int J Med Sci; Exercise combined with trimetazidine improves anti-fatal stress capacity through enhancing autophagy and heat shock protein 70 of myocardium in mice.; Authors - Lingjun Jiang
human	WB	Mol Cell Proteomics; A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications; Authors - Yuan-Qin Min