HSP70 Polyklonaler Antikörper

HSP70 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 25405-1-AP

Synonyme

Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1B, HSP70, HSP70 1/HSP70 2, HSP70 1B, HSP70 2, HSPA1, HSPA1A, HSPA1B

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Galerie der Validierungsdaten

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  WB analysis using 25405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25405-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25405-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25405-1-AP

  IP result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:5000) with HEK-293 cells lysate 2320 ug.

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  IP experiment of K-562 using 25405-1-AP

  IP Result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:1000) with K-562 cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

25405-1-AP bindet in WB, IP, IF, ELISA HSP70 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSP70 fusion protein Ag13700
• Vollständiger Name: heat shock 70kDa protein 1B
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC057397
• Gene symbol: HSPA1B
• Gene ID (NCBI): 3304
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1. What is Hsp70/HSP1A?

HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

2. FAQs for Hsp70

a. I cannot detect Hsp70 by western blotting

HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

a. What loading control can I use for cellular stress experiments with Hsp70?

Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Feedback inhibition of cAMP effector signaling by a chaperone-assisted ubiquitin system.; Authors - Laura Rinaldi
mouse	WB	Hum Mol Genet; Loss of Ten-Eleven Translocation 2 induces cardiac hypertrophy and fibrosis through modulating ERK signaling pathway.; Authors - Huikang Tao
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
human	WB	Mol Cell Biol; An Important Role for RPRD1B in the Heat Shock Response; Authors - Simona Cugusi
human	WB	Front Biosci (Landmark Ed); Exosomal miR-22-3p from Mesenchymal Stem Cells Inhibits the Epithelial-Mesenchymal Transition (EMT) of Melanoma Cells by Regulating LGALS1; Authors - Yong Chen
human	WB	Cancer Gene Ther; Loss of exosomal miR-200b-3p from hypoxia cancer-associated fibroblasts promotes tumorigenesis and reduces sensitivity to 5-Flourouracil in colorectal cancer via upregulation of ZEB1 and E2F3; Authors - Wenjing Gong