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HSP90 Polyklonaler Antikörper
HSP90 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (4)
Anwendung
WB, RIP, IP, IHC, IF, FC, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13171-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293-Zellen, C6-Zellen, HeLa-Zellen, Mausnierengewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattennierengewebe |
Erfolgreiche IP | K-562-Zellen, HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, Maushodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in FC | HeLa-Zellen, A549-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:20-1:200 |
Durchflusszytometrie (FC) | FC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
Produktinformation
13171-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA HSP90 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Fisch, Hausschwein, Huhn, Maus, Ratte, Rind |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | HSP90 fusion protein Ag3826 |
Vollständiger Name | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
Berechnetes Molekulargewicht | 853 aa, 90 kDa |
Beobachtetes Molekulargewicht | 90 kDa |
GenBank-Zugangsnummer | BC023006 |
Gene symbol | HSP90AA1 |
Gene ID (NCBI) | 3320 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for HSP90 antibody 13171-1-AP | Protokoll herunterladen |
IHC protocol for HSP90 antibody 13171-1-AP | Protokoll herunterladen |
IF protocol for HSP90 antibody 13171-1-AP | Protokoll herunterladen |
IP protocol for HSP90 antibody 13171-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Commun FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment | ||
Mol Cell SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism. | ||
Neuron UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. | ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Satyendra (Verified Customer) (07-06-2022) | Works very nicely with CEF, DF-1 and VeroE6 cells
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FH Guorong (Verified Customer) (03-22-2022) | Excellent performance with a specific band of expected size
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FH Ekaterini (Verified Customer) (02-16-2022) | The best antibody to use for loading control. Always clear and it is not affected by drug treatments.
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FH Xiaoping (Verified Customer) (10-22-2018) | This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.
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