HSP90 Monoklonaler Antikörper

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• KD/KO Validated

HSP90 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2D3D1

Kat-Nr. : 67450-1-Ig

Synonyme

HSP90AB1, 2D3D1, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta

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  WB analysis using 67450-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67450-1-Ig (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67450-1-Ig

  WB result of HSP90AB1 antibody (67450-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

67450-1-Ig bindet in WB, ELISA HSP90 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP90 fusion protein Ag28379
• Vollständiger Name: heat shock protein 90kDa alpha (cytosolic), class B member 1
• Berechnetes Molekulargewicht: 723 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83-90 kDa
• GenBank-Zugangsnummer: BC014485
• Gene symbol: HSP90AB1
• Gene ID (NCBI): 3326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Publikationen

Species	Application	Title
human	WB	Virol Sin; Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus; Authors - Bo Wang ; KD Validated