HUWE1 Polyklonaler Antikörper

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• KD/KO Validated

HUWE1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 19430-1-AP

Synonyme

ARF binding protein 1, ARF BP1, HECTH9, HSPC272, HUWE1, Ib772, KIAA0312, KIAA1578, Large structure of UREB1, LASU1, Mcl 1 ubiquitin ligase E3, MULE, URE B1, URE binding protein 1, UREB1

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Galerie der Validierungsdaten

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  WB analysis using 19430-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 19430-1-AP (HUWE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19430-1-AP

  WB result of HUWE1 antibody (19430-1-AP, 1:1000) with 293 cells by Dr. Emilia Galperin, University of Kentucky.

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  IHC staining of human lung cancer using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19430-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

19430-1-AP bindet in WB, IP, IHC, IF, ELISA HUWE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HUWE1 fusion protein Ag13763
• Vollständiger Name: HECT, UBA and WWE domain containing 1
• Berechnetes Molekulargewicht: 437 aa, 482 kDa
• Beobachtetes Molekulargewicht: 482 kDa
• GenBank-Zugangsnummer: BC002602
• Gene symbol: HUWE1
• Gene ID (NCBI): 10075
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).

Publikationen

Species	Application	Title
human	WB	Mol Cell; WNK1 is an assembly factor for the human ER membrane protein complex.; Authors - Tino Pleiner
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao ; KD Validated
human,mouse	WB	FASEB J; HUWE1 promotes EGFR ubiquitination and degradation to protect against renal tubulointerstitial fibrosis.; Authors - Qian Zhu
human	WB	J Cell Sci; Derlin-1 promotes ubiquitination and degradation of epithelial sodium channel (ENaC).; Authors - Hui You ; KD Validated
human	WB,IP	J Cell Sci; The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.; Authors - Patricia Wilson ; KD Validated
human	WB	Biochem Biophys Res Commun; NEDD4L-mediated LTF protein degradation limits ferroptosis.; Authors - Yuan Wang