• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Heavy chain of Rabbit IgG Monoklonaler Antikörper

Heavy chain of Rabbit IgG Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Kaninchen

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C9A5

Kat-Nr. : CL647-66467

Synonyme

Rabbit IgG, Heavy Chain

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated Heavy chain of Rabbi (CL647-66467)

    IF Staining of HeLa using CL647-66467

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 647-conjugated version of this antibody, CL647-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Heavy chain of Rabbi (CL647-66467)

    FC experiment of HeLa using CL647-66467

    1X10^6 HeLa cells were intracellularly stained with Beta Catenin Polyclonal antibody (51067-2-AP) and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5) (red), or Control Antibody and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-66467 bindet in IF/ICC, FC (Intra) Heavy chain of Rabbit IgG und zeigt Reaktivität mit Kaninchen

Getestete Reaktivität Kaninchen
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name Heavy chain of Rabbit IgG
Gene symbol
Gene ID (NCBI)
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This antibody specifically recognizes the heavy chain of Rabbit IgG.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 647 Heavy chain of Rabbit IgG antibody CL647-66467Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen