Heavy chain of Rabbit IgG Monoklonaler Antikörper

Heavy chain of Rabbit IgG Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Kaninchen und mehr (1)

Anwendung

WB, IP

Konjugation

HRP

Publikationen(7)

CloneNo.

1C9A5

Kat-Nr. : HRP-66467

Synonyme

Heavy Chain, Heavy Chain of Rabbit IgG, Rabbit IgG

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  WB analysis using HRP-66467

  Various serum/plasma lysate were subjected to SDS PAGE followed by western blot with HRP-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using HRP-66467

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. HRP-66467 (Heavy chain of Rabbit IgG antibody) as secondary antibody with dilution 1:15000. .

• 

  WB analysis of HeLa using HRP-66467

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, Kaninchen-Serumgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IP: See 1 publications below

Produktinformation

HRP-66467 bindet in WB, IP Heavy chain of Rabbit IgG und zeigt Reaktivität mit Kaninchen

• Getestete Reaktivität: Kaninchen
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Protein
• Vollständiger Name: Heavy chain of Rabbit IgG
• Konjugation: HRP
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This antibody specifically recognizes the heavy chain of Rabbit IgG. It can't detect the The heavy chain of other species. This antibody can be used as the secondary antibody which is specific to the heavy chain of Rabbit IgG.

Publikationen

Species	Application	Title
mouse	IP	FEBS J; NDRG2 alleviates photoreceptor apoptosis by regulating the STAT3/TIMP3/MMP pathway in mice with retinal degenerative disease; Authors - Tao Chen
	WB	Pharm Biol; Effects of total coumarins from Pileostegia tomentella on exosomal miRNA expression and angiogenesis in colorectal cancer cells; Authors - Ying Liu
		Ann Hematol; Licochalcone A decreases cancer cell proliferation and enhances ferroptosis in acute myeloid leukemia through suppressing the IGF2BP3/MDM2 cascade; Authors - Pingping Han
	WB	J Immunol; Homeostatic Macrophages Prevent Preterm Birth and Improve Neonatal Outcomes by Mitigating In Utero Sterile Inflammation in Mice; Authors - Valeria Garcia-Flores
	WB	Nitric Oxide; Hydrogen Sulfide Ameliorated Endothelial Dysfunction in hyperhomocysteinemia Rats: Mechanism of IRE1α/JNK pathway-mediated Autophagy; Authors - Yuan Gao
	WB	Curr Med Sci; USP19 Stabilizes TAK1 to Regulate High Glucose/Free Fatty Acid-induced Dysfunction in HK-2 Cells; Authors - Xiao-Hui Yan