ICAM2 Polyklonaler Antikörper

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• KD/KO Validated

ICAM2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10121-2-AP

Synonyme

CD102, ICAM 2, ICAM2

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 10121-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10121-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10121-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10121-2-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, Maushirngewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Produktinformation

10121-2-AP bindet in WB, IHC, ELISA ICAM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ICAM2 fusion protein Ag0165
• Vollständiger Name: intercellular adhesion molecule 2
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 55-80 kDa
• GenBank-Zugangsnummer: BC003097
• Gene symbol: ICAM2
• Gene ID (NCBI): 3384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.

Publikationen

Species	Application	Title
mouse	WB	Front Cell Infect Microbiol; Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion.; Authors - Song-Song Zou
human	WB,IHC	PLoS One; ICAM-2 expression mediates a membrane-actin link, confers a nonmetastatic phenotype and reflects favorable tumor stage or histology in neuroblastoma.; Authors - Yoon Karina Jin KJ ; KD Validated
human	IHC	J Ovarian Res; The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas; Authors - Xinxin Xu
human	IHC	Hum Pathol; Intercellular adhesion molecule 1/2 and E-selectin in plasma cell mastitis: immunohistochemical study of 35 cases.; Authors - Yu Dong