ICAM2 Polyklonaler Antikörper

ICAM2 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27322-1-AP

Synonyme

CD102, ICAM 2, ICAM2

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Galerie der Validierungsdaten

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  WB analysis using 27322-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27322-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 27322-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HUVEC using 27322-1-AP

  1X10^6 HUVEC cells were stained with 0.2 ug Anti-Human ICAM2 (27322-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HUVEC-Zellen, Jurkat-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in FC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27322-1-AP bindet in WB, FC, ELISA ICAM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ICAM2 fusion protein Ag25952
• Vollständiger Name: intercellular adhesion molecule 2
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 55-80 kDa
• GenBank-Zugangsnummer: BC003097
• Gene symbol: ICAM2
• Gene ID (NCBI): 3384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.