IDH2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IDH2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

43

Rezensionen (1)

Kat-Nr. : 15932-1-AP

Synonyme

ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase

---

Galerie der Validierungsdaten

• 

  WB analysis using 15932-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15932-1-AP

  WB result of IDH2 antibody (15932-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells.

• 

  WB analysis of mouse heart using 15932-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15932-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15932-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15932-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15932-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15932-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15932-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15932-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 15932-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15932-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15932-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15932-1-AP

  IP Result of anti-IDH2 (IP:15932-1-AP, 3ug; Detection:15932-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of human colon cancer using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Mausherzgewebe, Maushirngewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen, Rattenlebergewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 38 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15932-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA IDH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IDH2 fusion protein Ag8779
• Vollständiger Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 41-47 kDa
• GenBank-Zugangsnummer: BC009244
• Gene symbol: IDH2
• Gene ID (NCBI): 3418
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

Publikationen

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
pig	WB	Sci Total Environ; Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets; Authors - Xiaotong Wang
human	WB,IHC	Mol Syst Biol; STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.; Authors - Monika Oberhuber
human	WB	Cancer Res; SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth.; Authors - Xianghui Zou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

Verwandte Produkte

Aconitase 2 Polyclonal antibody

11134-1-AP

ACLY Polyclonal antibody

15421-1-AP

IDH3A Polyclonal antibody

15909-1-AP

GOT2 Polyclonal antibody

14800-1-AP

Peripherin Monoclonal antibody

66317-1-Ig