IDI1 Polyklonaler Antikörper

IDI1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 11166-2-AP

Synonyme

IDI1, IPP isomerase 1, IPP1, IPPI1

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Galerie der Validierungsdaten

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  WB analysis of L02 using 11166-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11166-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11166-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11166-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11166-2-AP

  IP Result of anti-IDI1 (IP:11166-2-AP, 4ug; Detection:11166-2-AP 1:300) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 11166-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11166-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11166-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11166-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, humanes Lebergewebe, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

11166-2-AP bindet in WB, IP, IHC, ELISA IDI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IDI1 fusion protein Ag1636
• Vollständiger Name: isopentenyl-diphosphate delta isomerase 1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC019227
• Gene symbol: IDI1
• Gene ID (NCBI): 3422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDI1(Isopentenyl-diphosphate Delta-isomerase 1) is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP). It belongs to the IPP isomerase type 1 family. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 26 kDa and 32 kDa.

Publikationen

Species	Application	Title
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	WB	PLoS One; Timosaponin AIII is preferentially cytotoxic to tumor cells through inhibition of mTOR and induction of ER stress.; Authors - King Frank W FW
mouse	WB	Curr Issues Mol Biol; Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.; Authors - Audrey Basque
human	WB	Proteomes; Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.; Authors - Henrick Horita
mouse	WB	Toxicol In Vitro; Sinapic and ferulic acid phenethyl esters increase the expression of steroidogenic genes in MA-10 tumor Leydig cells; Authors - Audrey Basque

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (12-20-2020)

working good for IHC at 1;200 dilution

• Applications: Immunohistochemistry
• Cell Tissue Type: mouse liver tissues and rat liver