Iduronate 2 sulfatase Monoklonaler Antikörper

Iduronate 2 sulfatase Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A3F9

Kat-Nr. : 66112-1-Ig

Synonyme

IDS, iduronate 2 sulfatase, Idursulfase

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Galerie der Validierungsdaten

• 

  WB analysis of human placenta using 66112-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66112-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66112-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66112-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66112-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66112-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66112-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

• 

  IHC staining of human liver using 66112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

• 

  IF Staining of HepG2 using 66112-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

• 

  IF Staining of HepG2 using 66112-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human placenta, Hausschwein-Hirngewebe, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66112-1-Ig bindet in WB, IHC, IF, ELISA Iduronate 2 sulfatase und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Iduronate 2 sulfatase fusion protein Ag19095
• Vollständiger Name: iduronate 2-sulfatase
• Berechnetes Molekulargewicht: 312aa,35 kDa; 550aa,62 kDa
• Beobachtetes Molekulargewicht: 76 kDa, 55 kDa
• GenBank-Zugangsnummer: BC006170
• Gene symbol: IDS
• Gene ID (NCBI): 3423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).