IFITM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IFITM1 Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Kat-Nr. : 11727-3-AP

Synonyme

9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 11727-3-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11727-3-AP (IFITM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11727-3-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11727-3-AP (IFITM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of ROS1728 using 11727-3-AP

  IP Result of anti-IFITM1 (IP:11727-3-AP, 4ug; Detection:11727-3-AP 1:500) with ROS1728 cells lysate 3200ug.

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  IHC staining of human lymphoma using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of K-562 using 11727-3-AP

  1X10^6 K-562 cells were stained with 0.2ug IFITM1 antibody (11727-3-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, K-562-Zellen
• Erfolgreiche IP: ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• FC: See 4 publications below

Produktinformation

11727-3-AP bindet in WB, IP, IHC, IF, FC, ELISA IFITM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ziege, Südliche Grünmeerkatze
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFITM1 fusion protein Ag2320
• Vollständiger Name: interferon induced transmembrane protein 1 (9-27)
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14-17 kDa
• GenBank-Zugangsnummer: BC000897
• Gene symbol: IFITM1
• Gene ID (NCBI): 8519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.

Publikationen

Species	Application	Title
human	WB	Nature; Mitotic progression following DNA damage enables pattern recognition within micronuclei.; Authors - Shane M Harding
human	FC	Nature; Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells.; Authors - Edward J Grow ; KD Validated
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB	Mol Ther; Type 1 Interferon Responses Underlie Tumor-Selective Replication of Oncolytic Measles Virus.; Authors - Sarah Aref
human	WB	Nat Commun; IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses; Authors - M. Clement
human	WB	Cell Rep; IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.; Authors - Jingyou Yu ; KD Validated

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