IFN-beta Rekombinanter Antikörper

IFN-beta Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240230B6

Kat-Nr. : CL594-98082

Synonyme

Ifnb1, 240230B6, Ifb, IFN beta, IFNB

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  FC experiment of mouse splenocytes using CL594-98082

  1x10^6 mouse splenocytes were intracellularly stained with CoraLite® Plus 488 Anti-Mouse CD19, and 0.25 ug CoraLite®594 Anti-Mouse IFN-beta Rabbit RecAb (CL594-98082, Clone: 240230B6) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of mouse splenocytes using CL594-98082

  1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse IFN-beta Rabbit RecAb (CL594-98082, Clone: 240230B6) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98082 bindet in FC (Intra) IFN-beta und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IFN-beta fusion protein Eg0660
• Vollständiger Name: interferon beta 1, fibroblast
• Berechnetes Molekulargewicht: 22KD
• GenBank-Zugangsnummer: NM_010510.1
• Gene symbol: Ifnb1
• Gene ID (NCBI): 15977
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interferon-beta (IFN-β) is a type I interferon, which is a group of signaling proteins made and released by host cells in response to the presence of several viruses, including double-stranded RNA produced by many viruses. IFN-β is a single protein species and is molecularly related to IFN-α subtypes, although it is antigenically distinct from them. It is mainly produced by fibroblasts and plays a crucial role in the innate immune response against viruses. IFN-β can inhibit viral replication by interfering with the virus's RNA or DNA, thus suppressing viral growth. It also enhances the cytotoxic activity of natural killer (NK) cells and promotes the expression of major histocompatibility complex (MHC) class I molecules.