IFN-gamma Rekombinanter Antikörper

IFN-gamma Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

6K20

Kat-Nr. : 98001-1-PBS

Synonyme

IFN Gamma, Ifng, interferon gamma, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFNG2

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• 

  FC experiment of wistar rat splenocytes using 98001-1-RR (same clone as 98001-1-PBS)

  1X10^6 unstimulated or ConA, PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Wistar rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IFN-gamma (98001-1-RR, Clone: 6K20) and PE-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody. Cells were co-stained with 0.25 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98001-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 98001-1-PBS

  1x10^6 unstimulated or ConA, PMA and ionomycin stimulated (in the presence of protein transport inhibitors) rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IFN-gamma (98001-1-PBS, Clone: 6K20) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with 0.25 ug FITC Plus Anti-Rat CD4 (OX-35) (FITC-65179, Clone: OX-35). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98001-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98001-1-RR against Rat IFN-gamma were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): ConA, PMA and ionomycin stimulated rat splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98001-1-PBS bindet in FC (Intra) IFN-gamma und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interferon gamma
• Berechnetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_138880
• Gene symbol: IFN-gamma
• Gene ID (NCBI): 25712
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. Stable for one year after shipment.

Hintergrundinformationen

Interferon-gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)