IFN-gamma Rekombinanter Antikörper

IFN-gamma Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241390B10

Kat-Nr. : 98187-1-RR

Synonyme

IFNG, 241390B10, IFG, IFN gamma, IFN γ

---

• 

  FC experiment of human PBMCs using 98187-1-RR

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IFN-gamma Rabbit RecAb (98187-1-RR, Clone:241390B10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using 98187-1-RR

  1x10^6 PMA and ionomycin treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IFN-gamma Rabbit Recombinant Antibody (98187-1-RR, Clone: 241390B10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IFN-gamma Rabbit Recombinant Antibody (98187-1-RR, Clone: 241390B10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98187-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98187-1-RR against Human IFN-gamma were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA and ionomycin treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98187-1-RR bindet in FC (Intra) IFN-gamma und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IFN-gamma fusion protein Eg0004
• Vollständiger Name: IFN gamma
• Berechnetes Molekulargewicht: 166 aa, 19 kDa
• GenBank-Zugangsnummer: BC070256
• Gene symbol: IFNG
• Gene ID (NCBI): 3458
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)