IFN-gamma Rekombinanter Antikörper

IFN-gamma Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240262C9

Kat-Nr. : CL594-98045

Synonyme

IFN gamma, Ifng, 240262C9, Ifg, IFN g

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  FC experiment of C57BL/6 Th1-polarized splenocytes using CL594-98045

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug CoraLite®594 Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (CL594-98045, Clone: 240262C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of C57BL/6 Th1-polarized splenocytes using CL594-98045

  1x10^6 PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (CL594-98045, Clone: 240262C9) (red) or 0.25 ug Isotype Control (blue). 1x10^6 untreated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (CL594-98045, Clone: 240262C9) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98045 bindet in FC (Intra) IFN-gamma und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IFN-gamma fusion protein Eg0334
• Vollständiger Name: interferon gamma
• Berechnetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC119063
• Gene symbol: IFN gamma
• Gene ID (NCBI): 15978
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)