IFN-gamma Monoklonaler Antikörper

IFN-gamma Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

CloneNo.

XMG1.2

Kat-Nr. : CL647-65153

Synonyme

IFN gamma, Ifng, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN g

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  FC experiment of C57BL/6 Th1-polarized splenocytes using CL647-65153

  1x10^6 PMA and ionomycin Stimulate C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Mouse IFN gamma (CL647-65153, Clone:XMG1.2), and 0.13 ug CoraLite® Plus 647 Rat IgG1 Isotype Control (HRPN) (CL647-65212, Clone: HRPN), and 0.13 ug PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA and ionomycin Stimulate C57BL/6 Th1-polarized splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

CL647-65153 bindet in FC (Intra) IFN-gamma und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Purified Recombinant Mouse IFN gamma
• Vollständiger Name: interferon gamma
• Berechnetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC119063
• Gene symbol: IFN gamma
• Gene ID (NCBI): 15978
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Publikationen

Species	Application	Title
	FC	Int J Mol Sci; Development of a Vaccine Candidate Based on Surface-Displayed Particles of Mycobacterium tuberculosis from the MTB39A Protein; Authors - Pu Wang