IFN-gamma Rekombinanter Antikörper

IFN-gamma Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

241390B10

Kat-Nr. : FITC-98187

Synonyme

IFNG, 241390B10, IFG, IFN gamma, IFN γ



Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)PMA and ionomycin treated human PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98187 bindet in FC (Intra) IFN-gamma und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IFN-gamma fusion protein Eg0004
Vollständiger Name IFN gamma
Berechnetes Molekulargewicht 166 aa, 19 kDa
GenBank-ZugangsnummerBC070256
Gene symbol IFNG
Gene ID (NCBI) 3458
Konjugation FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
FC protocol for FITC Plus IFN-gamma antibody FITC-98187Download protocol
STANDARD-PROTOKOLLE
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