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IFT140 Polyklonaler Antikörper
IFT140 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 17460-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhodengewebe | 
| Erfolgreiche IP | Rattenhodengewebe | 
| Erfolgreiche Detektion in IHC | Maushodengewebe,  humanes Skelettmuskelgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF-P | Maushodengewebe | 
| Erfolgreiche Detektion in IF/ICC | C2C12-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below | 
| WB | See 26 publications below | 
| IHC | See 4 publications below | 
| IF | See 63 publications below | 
| IP | See 1 publications below | 
| CoIP | See 1 publications below | 
Produktinformation
17460-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA IFT140 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Hund, Maus, Zebrafisch | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | IFT140 fusion protein Ag11119 | 
| Vollständiger Name | intraflagellar transport 140 homolog (Chlamydomonas) | 
| Berechnetes Molekulargewicht | 1462 aa, 165 kDa | 
| Beobachtetes Molekulargewicht | 150-165 kDa | 
| GenBank-Zugangsnummer | BC035577 | 
| Gene symbol | IFT140 | 
| Gene ID (NCBI) | 9742 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for IFT140 antibody 17460-1-AP | Protokoll herunterladen | 
| IHC protocol for IFT140 antibody 17460-1-AP | Protokoll herunterladenl | 
| IF protocol for IFT140 antibody 17460-1-AP | Protokoll herunterladen | 
| IP protocol for IFT140 antibody 17460-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Nat Genet Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. | ||
| Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. | ||
| Hepatology Biallelic mutations in TTC26 (IFT56) cause severe biliary ciliopathy in humans. | ||
| Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
| Nat Commun A distal centriolar protein network controls organelle maturation and asymmetry. | ||
| Nat Commun Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Charlotte (Verified Customer) (07-18-2024) | I tested the antibody in iF in LIGHT2 cells (commonly used to study Hh signalling). Unfortunately, I don't see the ciliary signal when overlapping IFT140 with ARL13B. This could be cell type specific or the conditions must be optimized to reveal it ? I used the standard iF protocol: PFA 4% 10 min cold methanol 5 min 1h 1% BSA in PBST 1/500 in blocking solution, O.N. at 4°C 5*5min PBST 1/500 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS 
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