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IFT140 Polyklonaler Antikörper

IFT140 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(81)

Kat-Nr. : 17460-1-AP

Synonyme

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhodengewebe
Erfolgreiche IPRattenhodengewebe
Erfolgreiche Detektion in IHCMaushodengewebe, humanes Skelettmuskelgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaushodengewebe
Erfolgreiche Detektion in IF/ICCC2C12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17460-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA IFT140 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hund, Maus, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IFT140 fusion protein Ag11119
Vollständiger Name intraflagellar transport 140 homolog (Chlamydomonas)
Berechnetes Molekulargewicht 1462 aa, 165 kDa
Beobachtetes Molekulargewicht150-165 kDa
GenBank-ZugangsnummerBC035577
Gene symbol IFT140
Gene ID (NCBI) 9742
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IFT140 antibody 17460-1-APProtokoll herunterladen
IHC protocol for IFT140 antibody 17460-1-APProtokoll herunterladenl
IF protocol for IFT140 antibody 17460-1-APProtokoll herunterladen
IP protocol for IFT140 antibody 17460-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Nat Genet

Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.

Authors - Carol-Anne Martin
mouseWB,IF

Cell

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Authors - Siew Cheng Phua

Hepatology

Biallelic mutations in TTC26 (IFT56) cause severe biliary ciliopathy in humans.

Authors - Ranad Shaheen
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
humanIF

Nat Commun

A distal centriolar protein network controls organelle maturation and asymmetry.

Authors - Lei Wang
mouseWB

Nat Commun

Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.

Authors - Linh T Vuong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Charlotte (Verified Customer) (07-18-2024)

I tested the antibody in iF in LIGHT2 cells (commonly used to study Hh signalling). Unfortunately, I don't see the ciliary signal when overlapping IFT140 with ARL13B. This could be cell type specific or the conditions must be optimized to reveal it ? I used the standard iF protocol: PFA 4% 10 min cold methanol 5 min 1h 1% BSA in PBST 1/500 in blocking solution, O.N. at 4°C 5*5min PBST 1/500 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: LIGHT2
IFT140 Antibody Immunofluorescence validation (1/500 dilution) in LIGHT2 (Cat no:17460-1-AP)