IFT140 Polyklonaler Antikörper

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• KD/KO Validated

IFT140 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

67

Kat-Nr. : 17460-1-AP

Synonyme

c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2

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Galerie der Validierungsdaten

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  WB analysis using 17460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17460-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 17460-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17460-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of rat testis using 17460-1-AP

  IP Result of anti-IFT140 (IP:17460-1-AP, 4ug; Detection:17460-1-AP 1:300) with rat testis tissue lysate 4400ug.

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  IHC staining of mouse testis using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of C2C12 using 17460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT140 antibody (17460-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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  IF Staining of mouse testis using 17460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using IFT140 antibody (17460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).

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  IF Staining of hTERT-RPE1 using 17460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT140 antibody (17460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C2C12-Zellen, hTERT-RPE1-Zellen, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 53 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17460-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA IFT140 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFT140 fusion protein Ag11119
• Vollständiger Name: intraflagellar transport 140 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 1462 aa, 165 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC035577
• Gene symbol: IFT140
• Gene ID (NCBI): 9742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Publikationen

Species	Application	Title
human	IF	Nat Genet; Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.; Authors - Carol-Anne Martin
mouse	WB,IF	Cell; Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.; Authors - Siew Cheng Phua
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; A distal centriolar protein network controls organelle maturation and asymmetry.; Authors - Lei Wang
mouse	WB	Nat Commun; Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.; Authors - Linh T Vuong
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet