IFT88 Rekombinanter Antikörper

IFT88 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230074E12

Kat-Nr. : 82886-2-RR

Synonyme

TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074E12

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  WB analysis using 82886-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82886-2-RR (IFT88 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 82886-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT88 antibody (82886-2-RR, Clone: 230074E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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  FC experiment of HEK-293 using 82886-2-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug IFT88 Recombinant antibody (82886-2-RR, Clone:230074E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82886-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82886-2-RR against Human IFT88 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: Starvation treated hTERT-RPE1 cells
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82886-2-RR bindet in WB, IF/ICC, ELISA IFT88 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IFT88 fusion protein Ag4980
• Vollständiger Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 88-95 kDa
• GenBank-Zugangsnummer: BC030776
• Gene symbol: IFT88
• Gene ID (NCBI): 8100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926).