IGF1R/CD221 Polyklonaler Antikörper

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• KD/KO Validated

IGF1R/CD221 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(57)

Rezensionen (1)

Kat-Nr. : 20254-1-AP

Synonyme

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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• 

  WB analysis of HeLa using 20254-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20254-1-AP (IGF1R beta chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20254-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20254-1-AP (IGF1R beta chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20254-1-AP

  IP result of anti-IGF1R (IP:20254-1-AP, 4ug; Detection:20254-1-AP 1:300) with HeLa cells lysate 2400ug.

• 

  IHC staining of human lung cancer using 20254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20254-1-AP (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 20254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20254-1-AP (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, L02-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 49 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

20254-1-AP bindet in WB, IHC, IF, IP, ELISA IGF1R/CD221 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: IGF I receptor
• Berechnetes Molekulargewicht: 155 kDa
• Beobachtetes Molekulargewicht: 100, 130 kDa
• GenBank-Zugangsnummer: NM_000875
• Gene symbol: IGF1R
• Gene ID (NCBI): 3480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

Publikationen

Species	Application	Title
human	IF	Sci Bull (Beijing); Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes; Authors - Jiangbing Xiang
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	WB	Mol Cancer; CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis.; Authors - Jinghan Hua
mouse	WB	J Cachexia Sarcopenia Muscle; Exogenous insulin-like growth factor 1 attenuates cisplatin-induced muscle atrophy in mice.; Authors - Hiroyasu Sakai
mouse	WB,IF	Cell Death Dis; Insulin-like growth factor 1 receptor mediates photoreceptor neuroprotection; Authors - Ammaji Rajala
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody had some faint non-specific bands in addition to a strong and specific IGF1R band.

• Applications: Western Blot
• Cell Tissue Type: Heart