IGF2BP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IGF2BP1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

59

Rezensionen (2)

Kat-Nr. : 22803-1-AP

Synonyme

CRD BP, CRDBP, IGF II mRNA binding protein 1, IGF2 mRNA binding protein 1, IGF2BP1, IMP 1, IMP1, VICKZ family member 1, VICKZ1, ZBP 1, ZBP1, Zip code binding protein 1, Zipcode binding protein 1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 22803-1-AP

  WB result of IGF2BP1 antibody (22803-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2BP1 transfected Jurkat cells.

• 

  WB analysis of Jurkat using 22803-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22803-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22803-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22803-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 22803-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 22803-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22803-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22803-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22803-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22803-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 22803-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 22803-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22803-1-AP

  IP Result of anti-IGF2BP1 (IP:22803-1-AP, 4ug; Detection:22803-1-AP 1:1000) with HEK-293 cells lysate 1480ug.

• 

  IHC staining of human lung cancer using 22803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22803-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22803-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 22803-1-AP

  Immunofluorescent analysis of A375 cells using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, HepG2-Zellen, Jurkat-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 18 publications below
• WB: See 49 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 9 publications below

Produktinformation

22803-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA IGF2BP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGF2BP1 fusion protein Ag18859
• Vollständiger Name: insulin-like growth factor 2 mRNA binding protein 1
• Berechnetes Molekulargewicht: 577 aa, 63 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC156957
• Gene symbol: IGF2BP1
• Gene ID (NCBI): 10642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF2BP1, also named as CRDBP, VICKZ1, ZBP1, is a 577 amino acid protein, which belongs to the RRM IMP/VICKZ family. IGF2BP1 can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. IGF2BP1 is mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus and also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta. IGF2BP1 is Expressed in various cancers, including testis and lung cancers, as well as kidney, prostate and trachea cancers. IGF2BP1 as RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. The calcualted molecular weight of IGF2BP1 is 63 kDa, but modified IGF2BP1 is about 65-70 kDa.

Publikationen

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun ; KD Validated
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza ; KD Validated
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
mouse	IHC,IF,WB	ACS Cent Sci; Allosteric Regulation of IGF2BP1 as a Novel Strategy for the Activation of Tumor Immune Microenvironment; Authors - Yang Liu
human	WB	Mol Cell; m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3; Authors - Ting Shan ; KD Validated
human	WB	Nat Commun; The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.; Authors - Zhe Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

tao (Verified Customer) (12-14-2022)

Immunofluorescence seems to work, but signal can be detected in the nucleus

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50

FH

Parisa (Verified Customer) (06-13-2022)

I used the antibody and it worked very well, It was sensitive and could detect the faint band that I expected in my IP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293