IGF2R-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IGF2R-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (2)

Kat-Nr. : 20253-1-AP

Synonyme

IGF2R, 300 kDa mannose 6-phosphate receptor, Cation-independent mannose-6-phosphate receptor, CD222, CI Man 6 P receptor

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  WB analysis using 20253-1-AP

  HepG2 cells lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20253-1-AP

  WB result of IGF2R-Specific antibody (20253-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2R-Specific transfected HepG2 cells.

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  WB analysis of L02 using 20253-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 20253-1-AP

  IP result of anti-IGF2R-Specific (IP:20253-1-AP, 4ug; Detection:20253-1-AP 1:500) with HepG2 cells lysate 3480ug.

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  IHC staining of human liver cancer using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of mouse colon using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 20253-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 20253-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IGF2R-Specific antibody (20253-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HepG2 using 20253-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IGF2R-Specific Polyclonal antibody (20253-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, L02-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Produktinformation

20253-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA IGF2R-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: insulin-like growth factor 2 receptor
• Berechnetes Molekulargewicht: 274 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: NM_000876
• Gene symbol: IGF2R
• Gene ID (NCBI): 3482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.

Publikationen

Species	Application	Title
human	WB	Hum Mol Genet; Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.; Authors - Yoshinori Tanaka
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
human	WB,IF	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human	WB	J Cancer; Trop2 binding IGF2R induces gefitinib resistance in NSCLC by remodeling the tumor microenvironment.; Authors - Xia Sun
human	WB	Life Sci Alliance; Identification and characterization of a membrane receptor that binds to human STC1.; Authors - Hin Ting Wan
rat	WB,IHC	J Sci Food Agric; Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation.; Authors - Shu Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tiphaine (Verified Customer) (06-20-2022)

Localization seems to be coherent

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: HeLa cells

IGF2R-Specific Antibody Immunofluorescence validation (1/100 dilution) in HeLa cells (Cat no:20253-1-AP)

FH

Q (Verified Customer) (03-22-2021)

A reliable antibody to detect endogenous IGF2R with WB. Although there are a few non specific bands at lower levels.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain

IGF2R-Specific Antibody Western Blot validation (1:2000 dilution) in mouse brain (Cat no:20253-1-AP)