IGFBP2 Polyklonaler Antikörper

IGFBP2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15699-1-AP

Synonyme

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 15699-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15699-1-AP (IGFBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15699-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15699-1-AP (IGFBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15699-1-AP

  Immunofluorescent analysis of HeLa cells using 15699-1-AP (IGFBP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15699-1-AP bindet in WB, IHC, IF, ELISA IGFBP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGFBP2 fusion protein Ag8327
• Vollständiger Name: IGF binding protein 2
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC004312
• Gene symbol: IGFBP2
• Gene ID (NCBI): 3485
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degarded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.