IgG Kappa Light Chain Polyklonaler Antikörper

IgG Kappa Light Chain Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, RIP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 14678-1-AP

Synonyme

IgG Kappa Light Chain, IGK, IGK-, IGKC, immunoglobulin kappa locus

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 14678-1-AP

  human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14678-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14678-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, humanes Lebergewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Plazenta-Gewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 5 publications below

Produktinformation

14678-1-AP bindet in WB, RIP, IHC, IF, ChIP, ELISA IgG Kappa Light Chain und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IgG Kappa Light Chain fusion protein Ag6389
• Vollständiger Name: immunoglobulin kappa locus
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC030813
• Gene symbol: IGK-
• Gene ID (NCBI): 50802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This antibody detects the light chain (Kappa) of human IgG.

Publikationen

Species	Application	Title
	IHC	Nat Commun; A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.; Authors - Christine Quach
	IHC	PLoS Genet; Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis.; Authors - Hyun-Jin Na
human	RIP	J Bone Miner Metab; Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes.; Authors - Lanjuan Shen
mouse	RIP	Mol Med Rep; Long non‑coding RNA XIST promotes cerebral ischemia/reperfusion injury by modulating miR‑27a‑3p/FOXO3 signaling.; Authors - Hua Zhang
mouse	RIP	J Exp Clin Cancer Res; RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner.; Authors - Dan Yu
human	IF	EBioMedicine; Integrating microarray-based spatial transcriptomics and single-cell RNA-sequencing reveals tissue architecture in esophageal squamous cell carcinoma; Authors - Wei Guo