IGSF2/CD101 Monoklonaler Antikörper

IGSF2/CD101 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E6A7

Kat-Nr. : 66333-1-Ig

Synonyme

IGSF2, CD101, Cell surface glycoprotein V7, EWI 101, EWI101

---

• 

  WB analysis using 66333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NR8383 using 66333-1-Ig

  NR8383 cells were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66333-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66333-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, NR8383 cells
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66333-1-Ig bindet in WB, IHC, ELISA IGSF2/CD101 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IGSF2/CD101 fusion protein Ag23297
• Vollständiger Name: immunoglobulin superfamily, member 2
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC136484
• Gene symbol: IGSF2
• Gene ID (NCBI): 9398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2.