IL-1 beta Rekombinanter Antikörper

IL-1 beta Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

PE Fluorescent Dye

CloneNo.

240902A3

Kat-Nr. : PE-FcA98142

Synonyme

Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta

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  FC experiment of human PBMCs using PE-FcA98142

  1x10^6 untreated or IFN gamma, LPS and Brefeldin A-treated human PBMCs were intracellularly stained with 5 ul PE Anti-Human IL-1 beta Rabbit RecAb (PE-FcA98142, Clone: 240902A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): IFN gamma, LPS and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-FcA98142 bindet in FC (Intra) IL-1 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-1 beta fusion protein Eg0286
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 269 aa, 31 kDa
• GenBank-Zugangsnummer: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.