IL-12/IL-23 p40 Rekombinanter Antikörper

IL-12/IL-23 p40 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241600F8

Kat-Nr. : 98277-1-RR

Synonyme

IL12B, 241600F8, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40

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  FC experiment of human PBMCs using 98277-1-RR

  1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using 98277-1-RR

  1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL12B Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were stained with 0.25 ug Anti-Human IL-12/IL-23 p40 Rabbit Recombinant Antibody (98277-1-RR, Clone: 241600F8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98277-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98277-1-RR against Human IL-12/IL-23 p40 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): IFN gamma, LPS and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98277-1-RR bindet in FC (Intra) IL-12/IL-23 p40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• Berechnetes Molekulargewicht: 328 aa, 37 kDa
• GenBank-Zugangsnummer: BC067498
• Gene symbol: IL12B
• Gene ID (NCBI): 3593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).