IL-12/IL-23 p40 Monoklonaler Antikörper

IL-12/IL-23 p40 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

C17.8

Kat-Nr. : CL488-65097

Synonyme

Il 12b, Il 12p40, IL-12/IL-23 p40, Il12b, Il-12b, Il12p40, Il-12p40, interleukin 12b, p40

---

Galerie der Validierungsdaten

• 

  FC experiment of Balb/c mouse peritoneal macrophages using CL488-65097

  IFN-γ primed Balb/c mouse peritoneal macrophages stimulated with LPS in the presence of a protein transport inhibitor were treated with fixation and permeabilization reagents and intracellularly stained with CoraLite® Plus 488-conjugated Rat IgG2a Isotype Control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse IL-12/IL-23 p40 (CL488-65097, Clone: C17.8) (right).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Balb/c peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65097 bindet in FC (Intra) IL-12/IL-23 p40 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHO-exprimiertes, rekombinantes Maus-IL-12 p70
• Vollständiger Name: interleukin 12b
• GenBank-Zugangsnummer: BC103608
• Gene symbol: Il12b
• Gene ID (NCBI): 16160
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C