IL-12/IL-23 p40 Monoklonaler Antikörper

IL-12/IL-23 p40 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

PE Fluorescent Dye

CloneNo.

C17.8

Kat-Nr. : PE-65097

Synonyme

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL-12/IL-23 p40, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

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Galerie der Validierungsdaten

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  FC experiment of Balb/c mouse peritoneal macrophages using PE-65097

  1X10^6 IFN-γ primed Balb/c mouse peritoneal macrophages stimulated with LPS in the presence of a protein transport inhibitor or unstimulated were intracellularly stained with 0.5 ug PE Anti-Human IL-12/IL-23 p40 (PE-65097, Clone: C17.8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Balb/c peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65097 bindet in FC (Intra) IL-12/IL-23 p40 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHO-exprimiertes, rekombinantes Maus-IL-12 p70
• Vollständiger Name: interleukin 12b
• GenBank-Zugangsnummer: BC103608
• Gene symbol: Il12b
• Gene ID (NCBI): 16160
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C