IL-13 Rekombinanter Antikörper

IL-13 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

2C14

Kat-Nr. : 98003-1-PBS

Synonyme

Il13, IL-13, interleukin 13, Interleukin-13, 2C14, Il 13

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  FC experiment of C57BL/6 Th2-polarized splenocytes using 98003-1-PBS

  1x10^6 unstimulated or PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-13 Rabbit Recombinant Antibody, PBS Only (98003-1-PBS, Clone:2C14) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes
• Erfolgreiche Detektion in FC: PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98003-1-PBS bindet in FC (Intra) IL-13 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 13
• Berechnetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: NM_008355
• Gene symbol: IL-13
• Gene ID (NCBI): 16163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. Stable for one year after shipment.

Hintergrundinformationen

Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin's disease.